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Peptide-affinity Purified Polyclonal Antibody to RAD17

Peptide-affinity Purified Polyclonal Antibody to RAD17

By: Stephen Jones Polyclonal Antibody to DTX1

Rad17, also known as 'cell cycle checkpoint protein RAD17,' is a nuclear protein belonging to the Rad17/RAD24 family and necessary for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 is known to have a weak ATPase activity and is required for binding to chromatin and also participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. It may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. Rad17 is a part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Four isoforms have been reported by X-ray irradiation. Overexpression of this protein is seen in various cancer cell lines and in colon carcinoma.
Antigen
A portion of human Rad17 protein containing a phosphorylated serine residue at position 645 was used as the immunogen. This product is antibody from the nonphosphorylated fraction of the immune response.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_f and CRA_d), rhesus monkey, chimpanzee and orangutan, 92% homologous in horse, cow and mouse and 85% homologous in dog and rat.

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Peptide-affinity Purified Polyclonal Antibody to RAD17

By: Stephen Jones